Myostatin

• Description:

  Myostatin is a TGF-beta family member that acts as an inhibitor of skeletal muscle growth. This muscle-specific cytokine interacts with Activin type I and type II receptors, and suppresses myoblast proliferation by arresting cell-cycle in the G1 phase. Suppression of Myostatin activity facilitates muscle formation and may be useful in reducing and/or preventing adiposity and type-2 diabetes. Myostatin activity can be blocked by the Activin-binding protein Follistatin, and by the propeptide of Myostatin. Recombinant human Myostatin is a 25.0 kDa protein consisting of two identical 109 amino acid polypeptides linked by a single disulfide bond.

• Synonyms:

  MSTN; GDF8; MSLHP

• NCBI Gene ID:

  2660

• UniProt:

  O14793

• Accession Number:

  NP_005250.1

• Accession Number mRNA:

  NM_005259.2

• Chromosomal Location:

  2q32.2

• Reactivity:

  Human/Mouse/Rat

• Cross Reactivity:

  Mouse, Rat, Human

• Sequence:

  DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected ED50 for this effect is 17.0-25.0 ng/ml.

• Length:

  109

• Form:

  Lyophilized

• Molecular Weight:

  25 kDa

• Host or Source:

  E. coli