MIA (Melanoma inhibitory activity)

• Description:

  MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3) -like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis. Recombinant human MIA is a 12.2 kDa globular protein containing 108 amino acid residues including two intramolecular disulfide bonds. .

• Synonyms:

  MIA; CD-RAP

• NCBI Gene ID:

  8190

• UniProt:

  Q16674

• Accession Number:

  NP_006524

• Accession Number mRNA:

  NM_006533

• Chromosomal Location:

  19q13.32-q13.33

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 μg/ml.

• Length:

  108

• Form:

  Lyophilized

• Molecular Weight:

  12.2 kDa

• Host or Source:

  E. coli