Leptin

• Description:

  Recombinant porcine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Recombinant porcine leptin was purified by proprietary chromatographic techniques, see Raver et al. Prot. Express. Purif. 19, 30-40 (2000) .

• Synonyms:

  Lep; ob; obese

• NCBI Gene ID:

  396832

• UniProt:

  Q29406

• Accession Number:

  NP_999005.1

• Accession Number mRNA:

  NM_213840.1

• Reactivity:

  Porcine

• Cross Reactivity:

  Porcine

• Sequence:

  AVPIWRVQDDTKTLIKTIVTRISDISHMQSVSSKQRVTGLDFIPGLHPVLSLSKMDQTLAIYQQILTSLPSRNVIQISNDLENLRDLLHLLASSKSCPLPQARALETLESLGGVLEASLYSTEVVALSRLQGALQDMLRQLDLSPGC

• Assay Protocol:

  It is recommended to reconstitute the lyophilized recombinant porcine leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98.0% as determined by Gel filtration and SDS-PAGE gel.

• Bioactivity:

  Recombinant porcine leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

• Length:

  146

• Form:

  Lyophilized

• Reconstitution:

  0.4% NaHCO3, pH 8-9

• Molecular Weight:

  16.0 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  AVIPW