ICOS Ligand / B7-H2 / ICOSLG Protein (hFc Tag)

• Description:

  Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. The present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production, and germinal center formation.

• Synonyms:

  B7-H2 Protein; B7h Protein; B7H2 Protein; B7RP-1 Protein; B7RP1 Protein; CD275 Protein; GL50 Protein; ICOS-L Protein; ICOSL Protein; IMD119 Protein; KIAA0653 Protein; LICOS Protein

• Accession Number:

  O75144-1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  HFc-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  ≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

• Bioactivity:

  Immobilized Recombinant Human ICOS Ligand / B7-H2 / ICOSLG Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human ICOS / AILIM / CD278 Protein (His & Fc Tag) . The EC50 is 8-30 ng/mL.

• Length:

  481

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  70 kDa (glycosylated monomer)

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells