CNTF

• Description:

  CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity. Recombinant human CNTF is synthesized as a 199 amino acid polypeptide (22.7 kDa) lacking a hydrophobic N-terminal signal for secretion

• Synonyms:

  CNTF; HCNTF

• NCBI Gene ID:

  1270

• UniProt:

  P26441

• Accession Number:

  NP_000605

• Accession Number mRNA:

  NM_000614

• Chromosomal Location:

  11q12.2

• Reactivity:

  Human

• Cross Reactivity:

  Hamster, Mouse, Rat, Frog, Human

• Sequence:

  MAFTEHSPLT PHRRDLCSRS IWLARKLRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to stimulate proliferation of human TF-1 cells using an ED50 concentration range of 50-150 ng/ml.

• Length:

  199

• Form:

  Lyophilized

• Buffer:

  5 mM Sodium Phosphate, pH 7.5 + 75 mM NaCl

• Molecular Weight:

  22.7 kDa

• Host or Source:

  E. coli