CDNF

• Description:

  CDNF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. CDNF and its structural homolog MANF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. CDNF and MANF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Recombinant human CDNF is an 18.5 kDa protein consisting of 162 amino acids including 8 cysteine residues.

• Synonyms:

  Cerebral dopamine neurotrophic factor, ARMETL1

• NCBI Gene ID:

  1270

• UniProt:

  Q49AH0

• Accession Number:

  NP_001025125

• Accession Number mRNA:

  NM_001029954

• Chromosomal Location:

  11q12.2

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  MQEAGGRPGA DCEVCKEFLN RFYKSLIDRG VNFSLDTIEK ELISFCLDTK GKENRLCYYL GAT kDaATKI LSEVTRPMSV HMPAMKICEK LKKLDSQICE LKYEKTLDLA SVDLRKMRVA ELKQILHSWG EECRACAEKT DYVNLIQELA PKYAATHPKT EL

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 μg/ml.

• Length:

  162

• Form:

  Lyophilized

• Molecular Weight:

  18.5 kDa

• Host or Source:

  E. coli