CD40L / CD154 / TNFSF5 Protein (ECD, hFc Tag)

• Description:

  The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC) . In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.

• Synonyms:

  CD154 Protein; CD40-L Protein; CD40L Protein; gp39 Protein; hCD40L Protein; HIGM1 Protein; IGM Protein; IMD3 Protein; T-BAM Protein; TNFSF5 Protein; TRAP Protein

• Accession Number:

  NP_000065.1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  HFc-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

• Bioactivity:

  1.Immobilized human CD40 at 2 μg/mL (100 μl/well) can bind human CD40L Fc Chimera with a linear range of 1.28-32 ng/mL. 2. Measured by its ability to induce CD40 pathway activation in a Luciferase receptor Assay System. The IC50 for this effect is 10-100 ng/mL. 3.Loaded Recombinant Human CD40 Protein on His1K Biosensor, can bind Recombinant Human CD40 Ligand Protein with an affinity constant of 5.88nM as determined in BLI assay (Sartorius Octet RH16) .

• Length:

  386

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, 10% Trehalose, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  47.8 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells