CD14, soluble

• Description:

  CD14 is a cell surface anchored glycoprotein that is expressed predominantly by monocytes and tissue macrophages. CD14 associates with MD-2 (LY-96) and TLR4 to form a receptor complex, which signals specifically in response to bacterial lipopolysaccharide (LPS) binding. The CD14/MD-2/TLR4 receptor complex signals via MyD88, TIRAP, and TRAF6, and ultimately activates NF-kappaB. CD14 also exists in a soluble form, designated as sCD14, which is capable of specifically binding LPS in the extracellular space. Recombinant sCD14 is a 331 amino acid glycoprotein comprising the extracellular portion of the CD14 receptor.

• Synonyms:

  Soluble CD14

• NCBI Gene ID:

  929

• UniProt:

  P08571

• Accession Number:

  NP_000582.1

• Accession Number mRNA:

  NM_000591.3

• Chromosomal Location:

  5q22-q32; 5q31.1

• Reactivity:

  Human

• Cross Reactivity:

  Mouse, Human

• Sequence:

  TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  >95% by SDS-PAGE & HPLC analysis

• Bioactivity:

  Determined by the dose dependent activation of NF-kappaB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/μl to 200 ng/μl. The NF-kappaB activation is enhanced when the assay is done in the presence of 0.25 ng/μl to 1.0 ng/μl bacterial LPS.

• Length:

  331

• Form:

  Lyophilized

• Molecular Weight:

  35.6 kDa

• Host or Source:

  HEK293 cells