Amphiregulin

• Description:

  Amphiregulin is an EGF-related growth factor that signals through the EGF/TGF-α receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant Murine Amphiregulin is an 11.3 kDa glycoprotein consisting of 98 amino acid residues.

• Synonyms:

  AR, AREG, Colorectum cell-derived growth factor (CRDGF)

• NCBI Gene ID:

  11839

• UniProt:

  P31955

• Accession Number:

  NP_033834.1

• Accession Number mRNA:

  NM_009704.4

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  SVRVEQVIKP KKNKTEGEKS TEKPKRKKKG GKNGKGRRNK KKKNPCTAKF QNFCIHGECR YIENLEVVTC NCHQDYFGER CGEKSMKTHS EDDKDLSK

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  ≥ 95% by SDS-PAGE gel and HPLC analyses

• Bioactivity:

  Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.

• Length:

  98

• Form:

  Lyophilized

• Molecular Weight:

  11.3 kDa

• Host or Source:

  E. coli