Recombinant Phospho-Histone H3 (Ser10) Monoclonal Antibody

• Background:

  Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H4 at Lys5 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.

• Abbreviation:

  Phospho-Histone H3 (Ser10)

• UniProt:

  P68431

• Host:

  Rabbit

• Reactivity:

  Human; Mouse

• Immunogen:

  Peptide. This information is proprietary to PTMab

• Target:

  Histone H; H3C; H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; H3C1; Histone H3.1; Histone H3/a; Histone H3/b; H3FL; HIST1H3B; H3FC; HIST1H3C; H3FB; HIST1H3D; H3FD; HIST1H3E; H3FI; HIST1H3F; H3FH; HIST1H3G; H3FK; HIST1H3H; H3FF; HIST1H3I; H3FJ; HIST1H3J; H3/A; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; Histone H3.1; Histone H3/a; Histone H3/b; H3 histone family; H31; H3a; histone 1; Histone cluster 1; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; member A

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A829

• Conjugation:

  Unconjugated

• Applications:

  WB; ChIP; IP

• Field of Research:

  Epigenetics and Nuclear Signaling; Isotype; Loading Controls

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:1000-1:5000; ChIP 6μg antibody/100μg chromatin; IP 1:50-1:100

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  17 kDa

• Observed Molecular Weight:

  17 kDa

• Isotype:

  IgG, κ