Recombinant Phospho-Histone H2A.X (Ser139) Monoclonal Antibody

• Background:

  Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

• Abbreviation:

  Histone H2A.X

• UniProt:

  P16104

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  A synthetic peptide corresponding to residues around (Ser139) of Human Phospho-Histone H2A.X

• Target:

  H2A.X; H2A/X; H2AX; Histone H2AX; H2AFX; gamma H2A.X; γH2AX

• Clonality:

  Monoclonal, Recombinant

• Clone:

  10A12

• Conjugation:

  Unconjugated

• Applications:

  WB

• Field of Research:

  Epigenetics and Nuclear Signaling

• Purification:

  Protein A

• Concentration:

  0.2 mg/mL

• Dilution:

  WB 1:1000-1:5000

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  15 kDa

• Observed Molecular Weight:

  15 kDa

• Isotype:

  IgG, κ