Recombinant Histone H3.3 Monoclonal Antibody

• Background:

  Histone H3.3 is a histone H3 variant that replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• Abbreviation:

  Histone H3.3

• UniProt:

  P84243

• Host:

  Rabbit

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  Peptide. This information is proprietary to PTMab.

• Target:

  H3F; histone H; H3F3A; H3.3A; H3F3; histone H3.3; family 3B (H3.3B) ; H3 histone; H3 histone family 3A; H3 histone family 3B; H3.3; H33; Histone H3.3Q; Histone H3.A; Histone H3.B; MGC87782; MGC87783; H3.3B; H3F3B; H3-3A; H3-3B; PP781

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A744

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC

• Field of Research:

  Epigenetics and Nuclear Signaling

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:1000; IHC 1:500-1:1000

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  15 kDa

• Observed Molecular Weight:

  15 kDa

• Isotype:

  IgG, κ