Recombinant beta II Tubulin Monoclonal Antibody

• Background:

  Beta-tubulin is a protein that forms the backbone of cells. Tubulin can be divided into several kinds of Tubulin, including α, β, γ, δ, and ε, among which α -tubulin and β -tubulin can form heterodimers and are the two most important Tubulin for microtubule formation. The molecular weights of α-tubulin and β-tubulin are 55kDa and 50kDa, respectively, and the actual detected bands are around 55kDa. As an internal reference protein, the protein levels of β tubulin generally do not change. Therefore, it has been widely used as a reference for the consistency of sample loading in Western Bloting and also for immunostaining to observe the microtubule structure of cells.

• Abbreviation:

  Beta II Tubulin

• UniProt:

  Q9BVA1

• Host:

  Rabbit

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  Recombinant human beta II Tubulin fragment

• Target:

  TUBB2B

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A592

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC; IP

• Field of Research:

  Isotype; Loading Controls; Tags & Cell Markers; Signal Transduction

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:2000; IHC 1:200-1:1000; IP 1:50-1:100

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  50 kDa

• Observed Molecular Weight:

  55 kDa

• Isotype:

  IgG, κ