Recombinant Phospho-Chk1 (Ser345) Monoclonal Antibody

• Background:

  The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

• Abbreviation:

  CHEK1

• UniProt:

  O14757

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide corresponding to residues around

• Target:

  CHK; CHEK; CHEK1; CHK1

• Clonality:

  Monoclonal

• Clone:

  11A8

• Conjugation:

  Unconjugated

• Applications:

  WB

• Field of Research:

  Epigenetics and Nuclear Signaling; Cancer

• Purification:

  Protein A

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:2000-1:20000

• Buffer:

  10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  54 kDa

• Observed Molecular Weight:

  56 kDa

• Isotype:

  IgG