Purified Anti-Mouse IL-10 Antibody[JES5-16E3]

• Background:

  Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.

• Abbreviation:

  IL-10

• UniProt:

  P18893

• Host:

  Rat

• Reactivity:

  Mouse

• Immunogen:

  Recombinant Mouse IL-10 protein

• Target:

  Interleukin; IL-; CSIF; If2a; Il-10; IL10A; TGIF; Interleukin-10; IL10; Cytokine synthesis inhibitory factor; N/A; CSIF; If2a; Il-10

• Clonality:

  Monoclonal

• Clone:

  JES5-16E3

• Conjugation:

  Unconjugated

• Applications:

  FCM

• Purification:

  >98%, Protein A/G purified

• Concentration:

  ≥ 1 mg/mL

• Dilution:

  66

• Buffer:

  PBS, pH 7.2. Contains 0.05% proclin 300.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

• Isotype:

  Rat IgG2b, κ