Mini Sample Human VEGF165 (Vascular Endothelial Growth Factor 165) ELISA Kit

• Abbreviation:

  VEGF165

• UniProt:

  P15692

• Cross Reactivity:

  This kit recognizes VEGF165 in samples.No significant cross-reactivity or interference between VEGF165 and analogues was observed.

• Target:

  MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

• Type:

  Sandwich-ELISA

• Applications:

  ELISA

• Field of Research:

  Cardiovascular; Signal Transduction; Cancer

• Assay Principle:

  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VEGF165 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VEGF165 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VEGF165 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 27 nm. The OD value is proportional to the concentration of VEGF165 . You can calculate the concentration of VEGF165 in the samples by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  109.38-7000 pg/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  65.63 pg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Detection Instrument:

  Microplate reader

• Species:

  Human

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Human VEGF165 concentrations in serum, plasma and other biological fluids.