CD56 (NCAM) Antibody

• Background:

  NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1) . NCAM mediates neuronal attachment, neurite extension and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms+ tumor, neuroblastoma and rhabdomyosarcoma (2) . CD56 and CD16 are commonly used to identify NK cells although some cells with the T cell markers CD3 and CD4 also express CD56 (3) .

• NCBI Gene ID:

  4684

• Swiss Prot:

  P13591

• Accession Number:

  NP_000606.3

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  CD56 (NCAM) antibody was raised against a peptide sequence around aa.850~854 (Q-T-K-E-N) derived from Human CD56 (NCAM) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  120 - 220 kDa

• Specificity:

  This antibody detects endogenous levels of total NCAM protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  NCAM1

• NCBI Official Name:

  Neural cell adhesion molecule 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Cunningham, B. A. et al. (1987) Science 236, 799-806. Seidenfaden, R. et al. (2003) Mol. Cell. Biol. 23, 5908-5918. Robertson, M. J. and Ritz, J. (1990) Blood 76, 2421-2438.

• Other Product Names:

  CD56, NCAM, MSK39, STK14A, NCAM1

• Tested Applications:

  WB

• Protein ID:

  94420689

• Physical Properties:

  Liquid