EGFR (phospho Thr678) Antibody

• Background:

  Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

• NCBI Gene ID:

  1956

• Swiss Prot:

  P00533

• Accession Number:

  NP_005219.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  EGFR (Phospho-Thr678) antibody was raised against a peptide sequence around phosphorylation site of threonine 678 (K-R-T (p) -L-R) derived from Human EGFR.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  175 kDa

• Specificity:

  This antibody detects endogenous level EGFR only when phosphorylated at threonine 678.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  EGFR

• NCBI Official Name:

  Epidermal growth factor receptor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Doherty JK, et al. (1999) Proc Natl Acad Sci U S A; 96 (19) : 10869-10874Wu TT, et al. (1998) Mol Biol Cell; 9 (7) : 1661-1674O’Rourke DM, et al. (1997) Proc Natl Acad Sci U S A; 94 (7) : 3250-3255Noguchi T, et al. (1994) Mol Cell Biol; 14 (10) : 6674-6682Kanner SB, et al. (1991) Mol Cell Biol; 11 (2) : 713-720

• Other Product Names:

  ERBB, HER1, mENA, ERBB1, PIG61, DBCN, LISX, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR

• Tested Applications:

  WB

• Protein ID:

  29725609

• Physical Properties:

  Liquid