CD45.1 Antibody [A20] (FITC)

• Background:

  The A20 monoclonal antibody reacts with the leukocytes of the CD45.1-expressing mouse strains (DA, SJL/J, RIII, and STS/A) . It does not cross-react with cells that express CD45.2. The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region’s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons. The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction. The A20 antibody inhibits some of the B lymphocytes responses, from CD45.1-expressing mice, to lipopolysaccharides and antigens.

• NCBI Gene ID:

  19264

• Swiss Prot:

  P06800

• Host:

  Mouse

• Reactivity:

  Mouse

• Clonality:

  Monoclonal

• Clone:

  A20

• Conjugation:

  FITC

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

• Concentration:

  Batch dependent

• Buffer:

  Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

• Fragment:

  Mouse IgG2a, kappa

• Specificity:

  The A20 monoclonal antibody reacts with the leukocytes of the CD45.1-expressing mouse strains (DA, SJL/J, RIII, and STS/A) .

• Symbol:

  Ptprc

• NCBI Official Name:

  Protein tyrosine phosphatase, receptor type, C

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Shen, F.W. (1981) Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances. G.J. H U. H and J.F. Kearney, editors. Elsevier/North-Holland, Amsterdam. pp. 2531.

• Background Reference 02:

  Yakura, H. I. D. E. T. A. K. A., Kawabata, I., Shen, F. W., Katagiri, M. (1986) . Selective inhibition of lipopolysaccharide-induced polyclonal IgG response by monoclonal Ly-5 antibody.The Journal of Immunology,136 (8), 2729-2733.

• Background Reference 03:

  Yakura, H., Shen, F. W., Bourcet, E., Boyse, E. A. (1983) . On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2.The Journal of experimental medicine,157 (4), 1077-1088.

• Other Product Names:

  Loc, B220, Cd45, L-CA, Ly-5, T200, CD45R, Lyt-4, Ptprc

• Tested Applications:

  Flow

• Physical Properties:

  Liquid