Keratin 8 (Ab 74) Antibody

• Background:

  Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

• NCBI Gene ID:

  3856

• Swiss Prot:

  P05787

• Accession Number:

  NP_002264.1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Keratin 8 (Ab-74) antibody was raised against a peptide sequence around aa.72~76 (L-L-S-P-L) derived from Human Keratin 8 (CK8) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  55 kDa

• Specificity:

  This antibody detects endogenous level of total Keratin 8 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  KRT8

• NCBI Official Name:

  Keratin 8

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Nakamichi I, et al. (2002) Mol Biol Cell; 13 (10) : 3441-3451. Prochasson P, et al. (2002) Nucleic Acids Res; 30 (15) : 3312-3322. Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91 (3) : 873-877. Thorey IS, et al. (1993) Mol Cell Biol; 13 (11) : 6742-6751.

• Other Product Names:

  K8, KO, CK8, CK-8, CYK8, K2C8, CARD2, HSTF1, CK 8, Cytokeratin endo A, K8

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  4504919

• Physical Properties:

  Liquid