EIF2a (Ab 51) Antibody

• Background :

  Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

• NCBI Gene ID :

  1965

• Swiss Prot :

  P05198

• Accession Number :

  NP_004085.1

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  EIF2a (Ab-51) antibody was raised against a peptide sequence around aa.49~53 (E-L-S-R-R) derived from Human eIF2α .

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration :

  1 mg/mL

• Buffer :

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight :

  38 kDa

• Specificity :

  This antibody detects endogenous level of total eIF2α protein.

• Applications Notes :

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol :

  EIF2S1

• NCBI Official Name :

  Eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Xavier Saelens, et, al. (2001) J. Biol. Chem; 276: 41620 - 41628. Hong-Li Wu, Yu-Hua Li, Yan-Hua Lin, et al. (2008) Salvianolic acid B protects human endothelial cells from oxidative stress damage: a possible protective role of glucose-regulated protein 78 induction Cardiovasc Res doi:10. 1093/cvr/cvn262.

• Other Product Names :

  EIF2, EIF-2, EIF2A, EIF-2A, EIF-2alpha, BHLHA39, DERMO1, Eukaryotic translation initiation factor 2 subunit alpha

• Tested Applications :

  WB, IHC, IF

• Protein ID :

  4758256

• Physical Properties :

  Liquid