PTEN (Ab 370) Antibody

• Background:

  Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3, 4, 5-trisphosphate, phosphatidylinositol 3, 4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1, 3, 4, 5-tetrakisphosphate with order of substrate preference in vitro PtdIns (3, 4, 5) P3 > PtdIns (3, 4) P2 > PtdIns3P > Ins (1, 3, 4, 5) P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.

• NCBI Gene ID:

  5728

• Swiss Prot:

  P60484

• Accession Number:

  NP_000305.3

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  PTEN (Ab-370) antibody was raised against a peptide sequence around aa.368~372 (D-V-S-D-N) derived from Human PTEN.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  54 kDa

• Specificity:

  This antibody detects endogenous level of total PTEN protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  PTEN

• NCBI Official Name:

  Phosphatase and tensin homolog

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Al-Khouri AM, et al. (2005) . J Biol Chem. 280 (42) : 35195-35202. Miller SJ, et al. (2002) . FEBS Lett. 528 (1-3) : 145-153. Torres J, et al. (2001) . J Biol Chem. 276 (2) : 993-998. Vazquez F, et al. (2000) . Mol Cell Biol. 20 (14) : 5010-5018.

• Other Product Names:

  BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, TYH, Mutated in multiple advanced cancers 1, Protein-tyrosine phosphatase PTEN

• Tested Applications:

  WB, IHC

• Protein ID:

  73765544

• Physical Properties:

  Liquid