CDK6 (phospho Tyr13) Antibody

• Background:

  Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.

• NCBI Gene ID:

  1021

• Swiss Prot:

  Q00534

• Accession Number:

  NP_001138778.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  CDK6 (phospho-Tyr13) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 13 (Q-Q-Y (p) -E-C) derived from Human CDK6.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  36 kDa

• Specificity:

  This antibody detects endogenous level of CDK6 only when phosphorylated at tyrosine 13.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  CDK6

• NCBI Official Name:

  Cyclin-dependent kinase 6

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625. Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681. Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659.

• Other Product Names:

  PLSTIRE, ERBB, ERBB1, HER1, Serine/threonine-protein kinase PLSTIRE

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  223718134

• Physical Properties:

  Liquid