FKHR (phospho Ser319) Antibody

• Background:

  FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q14

• NCBI Gene ID:

  2308

• Swiss Prot:

  Q12778

• Accession Number:

  NP_002006.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  FKHR (Phospho-Ser319) antibody was raised against a peptide sequence around phosphorylation site of serine 319 (T-S-S (p) -N-A) derived from Human FKHR/FOXO1A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  78 - 82 kDa

• Specificity:

  This antibody detects endogenous level of FKHR only when phosphorylated at serine 319.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  FOXO1

• NCBI Official Name:

  Forkhead box O1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rena G, et al. (2002) EMBO J 21 (9) : 2263-2271. Woods YL, et al. (2001) Biochem J355 (Pt 3) : 597-607. Rena G, et al. (2001) Biochem J 354 (Pt 3) : 605-612.

• Other Product Names:

  FKH1, FKHR, FOXO1A, MEK2, MKK2, PRKMK2, FOXO1

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  9257222

• Physical Properties:

  Liquid