CD47 Antibody

• Background:

  CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

• NCBI Gene ID:

  961

• Swiss Prot:

  Q08722

• Accession Number:

  Q08722

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CD47 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-315 amino acids from the C-terminal region of human CD47.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Immunology

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  35 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CD47

• NCBI Official Name:

  Leukocyte surface antigen CD47

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hatherley D., et.al., Mol. Cell 31:266-277 (2008) .

• Background Reference 02:

  Wollscheid B., et.al., Nat. Biotechnol. 27:378-386 (2009) .

• Other Product Names:

  Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47, CD47, MER6

• Tested Applications:

  WB

• Protein ID:

  1171879

• Physical Properties:

  Liquid