P-Glycoprotein Antibody

• Background:

  The membrane-associated ABCB1 protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White) . This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. ABCB1 is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

• NCBI Gene ID:

  5243

• Swiss Prot:

  P08183

• Accession Number:

  P08183

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This P-Glycoprotein (ABCB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 647-677 amino acids from the Central region of human P-Glycoprotein (ABCB1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  141 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  ABCB1

• NCBI Official Name:

  Multidrug resistance protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Saito, S., et al., J. Hum. Genet. 47 (1) :38-50 (2002) . Kerb, R., et al., Pharmacogenomics 2 (1) :51-64 (2001) . Cascorbi, I., et al., Clin. Pharmacol. Ther. 69 (3) :169-174 (2001) . Hoffmeyer, S., et al., Proc. Natl. Acad. Sci. U.S.A. 97 (7) :3473-3478 (2000) . Mickley, L.A., et al., Blood 91 (5) :1749-1756 (1998) .

• Other Product Names:

  Multidrug resistance protein 1, ATP-binding cassette sub-family B member 1, P-glycoprotein 1, CD243, ABCB1, MDR1, PGY1

• Tested Applications:

  WB

• Protein ID:

  238054374

• Physical Properties:

  Liquid