ART3 Antibody

• Background:

  Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI) -anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367-amino acid human ART3 protein has an estimated molecular mass of 41.5 kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35% amino acid identity.

• NCBI Gene ID:

  419

• Swiss Prot:

  Q13508

• Accession Number:

  Q13508

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ART3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-310 amino acids from the C-terminal region of human ART3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  44 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  ART3

• NCBI Official Name:

  Ecto-ADP-ribosyltransferase 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26) :16899-16903 (2002) .

• Background Reference 02:

  Koch-Nolte, F., et al., Genomics 39 (3) :370-376 (1997) .

• Background Reference 03:

  Levy, I., et al., FEBS Lett. 382 (3) :276-280 (1996) .

• Other Product Names:

  Ecto-ADP-ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, ARTC3, Mono (ADP-ribosyl) transferase 3, NAD (P) (+) --arginine ADP-ribosyltransferase 3, ART3, TMART

• Tested Applications:

  WB

• Protein ID:

  22261808

• Physical Properties:

  Liquid