RELA Antibody

• Background:

  NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1) /p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine) .

• NCBI Gene ID:

  5970

• Swiss Prot:

  Q04206

• Accession Number:

  Q04206

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This RELA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-60 amino acids from the N-terminal region of human RELA.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Obesity, Immunology, Cell Cycle, Cancer, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  60 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  RELA

• NCBI Official Name:

  Transcription factor p65

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pan, W.W., et al. J. Biol. Chem. 285 (45) :34348-34354 (2010)

• Background Reference 02:

  Tago, K., et al. J. Biol. Chem. 285 (40) :30622-30633 (2010)

• Background Reference 03:

  Park, J.S., et al. Oncol. Rep. 24 (3) :709-714 (2010)

• Background Reference 04:

  Yu, Z.H., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 26 (7) :650-652 (2010)

• Other Product Names:

  Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3

• Tested Applications:

  WB

• Protein ID:

  62906901

• Physical Properties:

  Liquid