VPS25 Antibody

• Background:

  VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]) .

• NCBI Gene ID:

  84313

• Swiss Prot:

  Q9BRG1

• Accession Number:

  Q9BRG1

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This VPS25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the C-terminal region of human VPS25.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  21 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  VPS25

• NCBI Official Name:

  Vacuolar protein-sorting-associated protein 25

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

• Background Reference 02:

  Talmud, P.J., et al. Am. J. Hum. Genet. 85 (5) :628-642 (2009)

• Background Reference 03:

  Im, Y.J., et al. Dev. Cell 17 (2) :234-243 (2009)

• Background Reference 04:

  Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)

• Other Product Names:

  Vacuolar protein-sorting-associated protein 25, hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, VPS25, DERP9, EAP20

• Tested Applications:

  WB

• Protein ID:

  73920459

• Physical Properties:

  Liquid