VPS25 Antibody

• Background :

  VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]) .

• NCBI Gene ID :

  84313

• Swiss Prot :

  Q9BRG1

• Accession Number :

  Q9BRG1

• Host :

  Rabbit

• Reactivity :

  Mouse

• Immunogen :

  This VPS25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the C-terminal region of human VPS25.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Signal Transduction

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Homology :

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  21 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  VPS25

• NCBI Official Name :

  Vacuolar protein-sorting-associated protein 25

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

• Background Reference 02 :

  Talmud, P.J., et al. Am. J. Hum. Genet. 85 (5) :628-642 (2009)

• Background Reference 03 :

  Im, Y.J., et al. Dev. Cell 17 (2) :234-243 (2009)

• Background Reference 04 :

  Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)

• Other Product Names :

  Vacuolar protein-sorting-associated protein 25, hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, VPS25, DERP9, EAP20

• Tested Applications :

  WB

• Protein ID :

  73920459

• Physical Properties :

  Liquid