EGF Antibody

• Background :

  This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.

• NCBI Gene ID :

  1950

• Swiss Prot :

  P01133

• Accession Number :

  P01133

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This EGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 940-967 amino acids from the C-terminal region of human EGF.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Cell Cycle, Signal Transduction

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  134 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  EGF

• NCBI Official Name :

  Pro-epidermal growth factor

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  De Diesbach, M.T., et al. Exp. Cell Res. 316 (19) :3239-3253 (2010)

• Background Reference 02 :

  Xu, Z., et al. Biochem. Biophys. Res. Commun. 401 (3) :376-381 (2010)

• Background Reference 03 :

  Lupien, M., et al. Genes Dev. 24 (19) :2219-2227 (2010)

• Background Reference 04 :

  Hommel, U., et al. J. Mol. Biol. 227 (1) :271-282 (1992)

• Other Product Names :

  Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone, EGF

• Tested Applications :

  WB

• Protein ID :

  251757262

• Physical Properties :

  Liquid