THOC6 Antibody

• Background:

  Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing-and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.

• NCBI Gene ID:

  79228

• Swiss Prot:

  Q86W42

• Accession Number:

  Q86W42

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This THOC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-123 amino acids from the Central region of human THOC6.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  38 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  THOC6

• NCBI Official Name:

  THO complex subunit 6 homolog

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

• Background Reference 02:

  Hosgood, H.D. III, et al. Occup Environ Med 66 (12) :848-853 (2009)

• Background Reference 03:

  Talmud, P.J., et al. Am. J. Hum. Genet. 85 (5) :628-642 (2009)

• Background Reference 04:

  Boyne, J.R., et al. PLoS Pathog. (10), E1000194 (2008) :

• Other Product Names:

  THO complex subunit 6 homolog, Functional spliceosome-associated protein 35, fSAP35, WD repeat-containing protein 58, THOC6, WDR58

• Tested Applications:

  WB

• Protein ID:

  74759455

• Physical Properties:

  Liquid