RNF144B Antibody

• Background:

  E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.

• NCBI Gene ID:

  255488

• Swiss Prot:

  Q7Z419

• Accession Number:

  Q7Z419

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This RNF144B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the Central region of human RNF144B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  34 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  RNF144B

• NCBI Official Name:

  E3 ubiquitin-protein ligase RNF144B

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sayan, B.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (29) :12877-12882 (2010)

• Background Reference 02:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 03:

  Benard, G., et al. EMBO J. 29 (8) :1458-1471 (2010)

• Background Reference 04:

  Markson, G., et al. Genome Res. 19 (10) :1905-1911 (2009)

• Other Product Names:

  E3 ubiquitin-protein ligase RNF144B, 632-, IBR domain-containing protein 2, RING finger protein 144B, p53-inducible RING finger protein, RNF144B, IBRDC2, P53RFP

• Tested Applications:

  WB

• Protein ID:

  57012811

• Physical Properties:

  Liquid