RASSF1 Antibody

• Background:

  This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

• NCBI Gene ID:

  11186

• Swiss Prot:

  Q9NS23

• Accession Number:

  Q9NS23

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This RASSF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human RASSF1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  39 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  RASSF1

• NCBI Official Name:

  Ras association domain-containing protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  El-Kalla, M., et al. Oncogene 29 (42) :5729-5740 (2010)

• Background Reference 02:

  Vardi, A., et al. In Vivo 24 (4) :393-400 (2010)

• Background Reference 03:

  Kim, J.H., et al. Virchows Arch. 457 (1) :35-42 (2010)

• Background Reference 04:

  Ahmed, I.A., et al. Cancer Genet. Cytogenet. 199 (2) :96-100 (2010)

• Other Product Names:

  Ras association domain-containing protein 1, RASSF1 {ECO:0000312|EMBL:AAF351282}

• Tested Applications:

  WB

• Protein ID:

  50401686

• Physical Properties:

  Liquid