SEMA4A Antibody

• Background:

  This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF) -mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10) . Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

• NCBI Gene ID:

  64218

• Swiss Prot:

  Q9H3S1

• Accession Number:

  Q9H3S1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This SEMA4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the N-terminal region of human SEMA4A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  84 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:500

• Symbol:

  SEMA4A

• NCBI Official Name:

  Semaphorin-4A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Davila, S., et al. Genes Immun. 11 (3) :232-238 (2010)

• Background Reference 02:

  Schmidt-Kastner, R., et al. Mol. Vis. 14, 125-135 (2008) :

• Background Reference 03:

  Toyofuku, T., et al. EMBO J. 26 (5) :1373-1384 (2007)

• Background Reference 04:

  Abid, A., et al. J. Med. Genet. 43 (4) :378-381 (2006)

• Other Product Names:

  Semaphorin-4A, Semaphorin-B, Sema B, SEMA4A, SEMAB, SEMB

• Tested Applications:

  WB

• Protein ID:

  29840871

• Physical Properties:

  Liquid