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MED16 Antibody

CAT: 0223-58-651Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-58-651Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
NCBI Gene ID
10025
Swiss Prot
Q9Y2X0
Accession Number
Q9Y2X0
Host
Rabbit
Reactivity
Human
Immunogen
This MED16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human MED16.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
97 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
MED16
NCBI Official Name
Mediator of RNA polymerase II transcription subunit 16
NCBI Organism
Homo sapiens
Background Reference 01
Sato, S., et al. Mol. Cell 14 (5) :685-691 (2004)
Background Reference 02
Tomomori-Sato, C., et al. J. Biol. Chem. 279 (7) :5846-5851 (2004)
Background Reference 03
Wang, Q., et al. J. Biol. Chem. 277 (45) :42852-42858 (2002)
Background Reference 04
Kang, Y.K., et al. Proc. Natl. Acad. Sci. U.S.A. 99 (5) :2642-2647 (2002)
Other Product Names
Mediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Trap95, Vitamin D3 receptor-interacting protein complex 92 kDa component, DRIP92, MED16, DRIP92 {ECO:0000312|EMBL:AAD310871}, THRAP5
Tested Applications
WB
Protein ID
62511180
Physical Properties
Liquid

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