SUPT5H Antibody

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

6829

O00267

O00267

Human

This SUPT5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-424 amino acids from the Central region of human SUPT5H.

Unconjugated

Primary Antibodies

Other

This antibody is purified through a protein A column, followed by peptide affinity purification.

Batch dependent

Predicted species reactivity based on immunogen sequence: Chicken, Mouse

Supplied in PBS with 0.09% (W/V) sodium azide.

None

Blue Ice

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

121 kDa

Rabbit Ig

For WB starting dilution is: 1:1000

SUPT5H

Transcription elongation factor SPT5

Homo sapiens

Wenzel, S., et al. Biochem. J. 425 (2) :373-380 (2010)

Chen, Y., et al. Genes Dev. 23 (23) :2765-2777 (2009)

Komori, T., et al. Genes Cells 14 (3) :343-354 (2009)

Chen, H., et al. PLoS ONE (9), E6918 (2009) :

Transcription elongation factor SPT5, hSPT5, DRB sensitivity-inducing factor 160 kDa subunit, DSIF p160, DRB sensitivity-inducing factor large subunit, DSIF large subunit, Tat-cotransactivator 1 protein, Tat-CT1 protein, SUPT5H, SPT5, SPT5H

WB