EIF3A Antibody

• Background:

  Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

• NCBI Gene ID:

  8661

• Swiss Prot:

  Q14152

• Accession Number:

  Q14152

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This EIF3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1219-1247 amino acids from the C-terminal region of human EIF3A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  167 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  EIF3A

• NCBI Official Name:

  Eukaryotic translation initiation factor 3 subunit A {ECO:0000255|HAMAP-Rule:MF_03000}

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Enchev, R.I., et al. Structure 18 (4) :518-527 (2010)

• Background Reference 02:

  Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19 (1) :251-257 (2010)

• Background Reference 03:

  Dong, Z., et al. Exp. Cell Res. 315 (11) :1889-1894 (2009)

• Background Reference 04:

  Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105 (47) :18139-18144 (2008)

• Other Product Names:

  Eukaryotic translation initiation factor 3 subunit A {ECO:0000255|HAMAP-Rule:MF_03000}, eIF3a {ECO:0000255|HAMAP-Rule:MF_03000}, Eukaryotic translation initiation factor 3 subunit 10 {ECO:0000255|HAMAP-Rule:MF_03000}, eIF-3-theta {ECO:0000255|HAMAP-Rule:MF_03000}, eIF3 p167, eIF3 p180, eIF3 p185, EIF3A {ECO:0000255|HAMAP-Rule:MF_03000}

• Tested Applications:

  WB, IHC-P

• Protein ID:

  6685537

• Physical Properties:

  Liquid