HDAC10 Antibody

• Background:

  Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1) . Eight members of HDAC family have been identified in the past several years (2,3) . These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3) . These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo. HDAC8 interacts with PEPB2-MYH11, a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11 produced by the inversion Inv (16) (p13q22), a translocation associated with acute myeloid leukemia of M4EO subtype. The PEPB2-MYH1 fusion protein also interacts with RUNX1, a well known transcriptional regulator, suggesting that the interaction with HDAC8 may participate to convert RUNX1 into a constitutive transcriptional repressor.

• NCBI Gene ID:

  83933

• Swiss Prot:

  Q969S8

• Accession Number:

  Q969S8

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This HDAC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human HDAC10.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  71 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  HDAC10

• NCBI Official Name:

  Histone deacetylase 10

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Keedy, K.S. et al.Virol. May; 83 (10) : 4749?756 (2009) .

• Background Reference 02:

  Tong, J.J., et al., Nucleic Acids Res. 30 (5) :1114-1123 (2002) .

• Background Reference 03:

  Fischer, D.D., et al., J. Biol. Chem. 277 (8) :6656-6666 (2002) .

• Background Reference 04:

  Guardiola, A.R., et al., J. Biol. Chem. 277 (5) :3350-3356 (2002) .

• Other Product Names:

  Histone deacetylase 10, HD10, HDAC10

• Tested Applications:

  WB

• Protein ID:

  27734403

• Physical Properties:

  Liquid