Glial Fibrillary Acidic Protein Antibody

• Background:

  Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers.

• NCBI Gene ID:

  281189

• Swiss Prot:

  Q28115

• Accession Number:

  Q28115

• Host:

  Chicken

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant and purified bovine GFAP.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Total IgY fraction

• Concentration:

  Batch dependent

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Glial Fibrillary Acidic Protein antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  50

• Specificity:

  Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule.

• Symbol:

  GFAP

• NCBI Official Name:

  Glial fibrillary acidic protein

• NCBI Organism:

  Bos taurus

• Background Reference 01:

  Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 (1972) .

• Background Reference 02:

  Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE and Messing A. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 (2001)

• Tested Applications:

  WB, IHC

• Protein ID:

  143811396

• Physical Properties:

  Liquid