GAT-1 Antibody

• Background:

  Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995) . GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003) .

• NCBI Gene ID:

  79212

• Swiss Prot:

  P23978

• Accession Number:

  P23978

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  Peptide corresponding to amino acid residues from the C-terminal region of rat GAT-1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  GAT-1 antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  67

• Applications Notes:

  The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with mouse tissue based on the fact that this species has 100% homology with the amino acid sequence used as antigen.

• Symbol:

  Slc6a1

• NCBI Official Name:

  Solute carrier family 6 (neurotransmitter transporter), member 1

• NCBI Organism:

  Rattus norvegicus

• Background Reference 01:

  Minelli A, Brecha NC, Karschin C, DeBiasi S, Conti F (1995) GAT-1, a high-affinity GABA plasma membrana transporter, is localizad to neurons and astroglia int he cerebral cortex. J. Neurosci. 15 (11) :7734-7746.

• Background Reference 02:

  Zhao WJ, Ma YH, Fei J, Mei ZT, Guo LH (2003) Increase in drug-induced seizure susceptibility of transgenic mice overexpressing GABA transporter 1. Acta Pharmacol. Sin. 24 (10) :991-5.

• Tested Applications:

  WB, IHC

• Protein ID:

  128609

• Physical Properties:

  Liquid