VASP Antibody

• Background:

  VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. VASP is an actin-associated protein involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of Listeria monocytogenes in host cells. It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients. Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.

• NCBI Gene ID:

  7408

• Swiss Prot:

  P50552

• Accession Number:

  4507869

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human VASP fused to a His-tag.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Immunology, Innate Immunity

• Concentration:

  Batch dependent

• Buffer:

  Liquid. Crude serum containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 1 year after receipt when stored at -20˚ C.

• Specificity:

  Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of human VASP. Does not cross-react with mouse or rat VASP.

• Applications Notes:

  ELISA: (direct or indirect: 1:2,000-1:5,000) . Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.

• Symbol:

  VASP

• NCBI Official Name:

  Vasodilator-stimulated phosphoprotein

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Vasodilator-stimulated Phosphoprotein

• Tested Applications:

  ICC, IP, WB

• Protein ID:

  NP_003361.1

• Physical Properties:

  Liquid