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VASP Antibody

CAT: 0223-36-349Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-36-349Size:100 µL
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Background
VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. VASP is an actin-associated protein involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of Listeria monocytogenes in host cells. It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients. Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.
NCBI Gene ID
7408
Swiss Prot
P50552
Accession Number
4507869
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human VASP fused to a His-tag.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Immunology, Innate Immunity
Concentration
Batch dependent
Buffer
Liquid. Crude serum containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 1 year after receipt when stored at -20˚ C.
Specificity
Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of human VASP. Does not cross-react with mouse or rat VASP.
Applications Notes
ELISA: (direct or indirect: 1:2,000-1:5,000) . Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.
Symbol
VASP
NCBI Official Name
Vasodilator-stimulated phosphoprotein
NCBI Organism
Homo sapiens
Other Product Names
Vasodilator-stimulated Phosphoprotein
Tested Applications
ICC, IP, WB
Protein ID
NP_003361.1
Physical Properties
Liquid

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