IL-33 Antibody

• Background :

  Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3) /ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

• NCBI Gene ID :

  77125

• Swiss Prot :

  Q8BVZ5

• Accession Number :

  68565902

• Host :

  Rabbit

• Reactivity :

  Mouse

• Immunogen :

  Recombinant mouse IL-33.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Innate Immunity

• Concentration :

  1 mg/ml

• Buffer :

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Stable for at least 6 months after receipt when stored at -20˚ C.

• Specificity :

  Recognizes mouse IL-33. Detects a band of ~18kDa by Western blot. Does not cross-react with human IL-33.

• Applications Notes :

  ELISA: (direct and indirect: 1:2,000-1:5,000) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

• Symbol :

  Il33

• NCBI Official Name :

  Interleukin 33

• NCBI Organism :

  Mus musculus

• Other Product Names :

  Interleukin-33; IL-1F11; NF-HEV

• Tested Applications :

  ELISA, WB

• Protein ID :

  Q8BVZ5

• Physical Properties :

  Liquid