Stearoyl-CoA Desaturase-1 Antibody

• Background:

  Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The enzyme is constitutively expressed in adipose tissue and is induced in liver in response to fat free, high carbohydrate diet. Several SCD isoforms (SCD1, -2, and -3) exist in the mouse. Mice with a targeted disruption of the SCD1 isoform had reduced body adiposity, increased insulin sensitivity, and resistance to diet-induced weight gain. The protection from obesity involved increased energy expenditure and increased oxygen consumption. SCD1 deficiency shows activation of lipid oxidation in addition to reduced triglyceride synthesis and storage.

• NCBI Gene ID:

  20249

• Swiss Prot:

  P13516

• Accession Number:

  21431735

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  Synthetic peptide corresponding to aa 21-33 (A21PPSGNEREKVKT33) of mouse SCD1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity

• Concentration:

  1 mg/ml

• Buffer:

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 6 months after receipt when stored at -20˚ C.

• Specificity:

  Recognizes mouse SCD1. Detects a band of ~41kDa by Western Blot.

• Applications Notes:

  ELISA: (direct and indirect: 1:2,000-1:5,000) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

• Symbol:

  Scd1

• NCBI Official Name:

  Stearoyl-Coenzyme A desaturase 1

• NCBI Organism:

  Mus musculus

• Other Product Names:

  SCD1; Acyl-CoA Desaturase 1; Fatty Acid Desaturase 1

• Tested Applications:

  ELISA, WB

• Protein ID:

  P13516

• Physical Properties:

  Liquid