Biotin Antibody [SPM375]

• Background:

  It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

• Swiss Prot:

  NotApplicable

• Host:

  Mouse

• Reactivity:

  All species

• Immunogen:

  Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.

• Clonality:

  Monoclonal

• Clone:

  SPM375

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1

• Applications Notes:

  1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

• Tested Applications:

  Flow, IF, WB, IHC, Functional Assays, CM

• Physical Properties:

  Liquid