Calponin Antibody [CLPN5-1]

• Background:

  Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

• NCBI Gene ID:

  1264

• Swiss Prot:

  P51911

• Host:

  Mouse

• Reactivity:

  Human, Rat

• Immunogen:

  Recombinant human protein was used as the immunogen for the Calponin antibody.

• Clonality:

  Monoclonal

• Clone:

  CLPN5-1

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  CNN1

• NCBI Official Name:

  Calponin 1, basic, smooth muscle

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  CNN1, Basic calponin, Calponin, Calponin-1, Calponins, basic, Calponin H1, smooth muscle, SMCC, Sm-Calp

• Tested Applications:

  IF, IHC-P

• Physical Properties:

  Liquid