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Mitochondrial Antibody [AE-1]

CAT: 0223-33-547Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0223-33-547Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
This mAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Swiss Prot
NotKnown
Host
Mouse
Reactivity
Human
Immunogen
A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.
Clonality
Monoclonal
Clone
AE-1
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Obesity
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Fragment
IgG1, kappa
Specificity
Does not react with mouse and rat
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
ELISA, IF, WB, IHC-P
Physical Properties
Liquid

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