Cytochrome C Antibody [6H2.B4]

• Background:

  Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32) . Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

• NCBI Gene ID:

  54205

• Swiss Prot:

  P99999

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Rat full-length protein was used as the immunogen for the Cytochrome C antibody.

• Clonality:

  Monoclonal

• Clone:

  6H2.B4

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Symbol:

  CYCS

• NCBI Official Name:

  Cytochrome c, somatic

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic

• Tested Applications:

  WB, IP, Flow, IF

• Physical Properties:

  Liquid