Glycophorin A Antibody [GYPA/280]

• Background:

  Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA) . It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

• NCBI Gene ID:

  2993

• Swiss Prot:

  P02724

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human GPA protein was used as the immunogen for the Glycophorin A antibody.

• Clonality:

  Monoclonal

• Clone:

  GYPA/280

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  GYPA

• NCBI Official Name:

  Glycophorin A (MNS blood group)

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  GYPA, glycophorin A (includes MN blood group), HGNC:4702, GPA, GPErik, GPSAT, HGpMiV, HGpMiX, HGpMiXI, HGpSta (C), MN, MNS, Mi.V glycoprotein (24 AA), erythroid-lineage-specific membrane sialoglycoprotein, glycophorin A, glycophorin Er

• Tested Applications:

  WB, Flow, IF, IHC-P

• Physical Properties:

  Liquid