Golgi Marker Antibody [GLG1/970]

• Background:

  This mAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils) . Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This mAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

• NCBI Gene ID:

  2734

• Swiss Prot:

  Q92896

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  The Golgi fraction from human liver cells was used as the immunogen for the Golgi Marker antibody.

• Clonality:

  Monoclonal

• Clone:

  GLG1/970

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Specificity:

  Does not react with mouse and rat

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  GLG1

• NCBI Official Name:

  Golgi glycoprotein 1

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Golgi apparatus protein 1, CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand 1, ESL-1, Golgi sialoglycoprotein MG-160, GLG1, CFR1, ESL1, MG160

• Tested Applications:

  Flow, IF, IHC-P, ICC, WB

• Physical Properties:

  Liquid