Cytokeratin 10 Antibody [LH2]

• Background:

  This antibody recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10) . cytokeratin 10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of cytokeratin 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, cytokeratin 10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), cytokeratin 10 is detected very frequently. Expression of cytokeratin 10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

• NCBI Gene ID:

  3858

• Swiss Prot:

  P13645

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  Skin extract from a human psoriasis patient was used as the immunogen for this Cytokeratin 10 antibody.

• Clonality:

  Monoclonal

• Clone:

  LH2

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8-9, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  KRT10

• NCBI Official Name:

  Keratin 10

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP

• Tested Applications:

  Flow, IHC, IF

• Physical Properties:

  Liquid